Cleveland Heartlab

Lipid Panel

CPT Code: 80061

Included Tests: Total Cholesterol, Calculated LDL-C, Direct HDL-C, Triglycerides

Order Code: C906

Synonyms:  none

Sample Type: Serum

Minimum Volume: 0.5 ml

Tube Type: Tiger top tube

Patient Preparation: Patient must be fasting 12 hours prior to being drawn.
 
Collection:
 
1. Draw sample and gently invert 5 times (DO NOT SHAKE)
2. Let the blood clot for 30 minutes
3. Centrifuge sample at 1300 rcf for 30 minutes

Storage Instructions:
Cleveland HeartLab recommends that the sample be stored at 4°C and shipped same day as draw.  The sample is stable for 6 days at 4°C.

Causes for Rejection: Samples will be rejected if there is gross hemolysis or if the sample is improperly labeled

Use: To evaluate hyperlipidemia as an indicator of heart disease

Methodology: See individual tests

Additional Information: none
 
 

Total Cholesterol

CPT Code: 82465

Order Code: C117

Synonyms: Chol

Sample Type: Serum

Minimum Volume:
1.0 ml

Tube Type:
Tiger top tube

Patient Preparation: Fasting or non-fasting sample may be used.

Collection:

1. Draw sample and gently invert 5 times (DO NOT SHAKE)
2. Let the blood clot for 30 minutes
3. Centrifuge sample at 1300 rdf for 30 minutes

Storage Instructions: Cleveland HeartLab recommends that sample be shipped same day as draw.  The sample is stable for 6 days at 4°C.

Causes for Rejection:   Samples will be rejected if improperly labeled.

Use: Cholesterol is a steroid with a secondary hydroxyl group in the C3 position. It is synthesized in many types of tissue, but particularly in the liver and intestinal wall. Approximately three quarters of cholesterol is newly synthesized and a quarter originates from dietary intake. Cholesterol assays are used for screening for atherosclerotic risk and in the diagnosis and treatment of disorders involving elevated cholesterol levels as well as lipid and lipoprotein metabolic disorders.

Methodology: Enzymatic Photometric

Goal Value:  <200 mg/dL

Additional Information: Total cholesterol can be used to evaluate hypo- or hyperlipidemia
 

Calculated LDL-C

Calculation to determine LDL-C using a standard lipid panel:
Total cholesterol-Direct HDL cholesterol-(Triglycerides/5)

Goal Value:  <100 mg/dL
 

Direct HDL-C 

CPT Code: 83718

Order Code: C118

Synonyms: Alpha- Lipoprotein Cholesterol, HDL-C

Sample Type: Serum

Minimum Volume: 1.0 ml

Tube Type: Tiger top tube

Patient Preparation: Fasting or non-fasting sample may be used.

Collection:
1. Draw sample and gently invert 5 times (DO NOT SHAKE)
2. Let the blood clot for 30 minutes
3. Centrifuge sample at 1300 rcf for 30 minutes

Storage Instructions: Cleveland HeartLab recommends that the sample be shipped same day as draw.  The sample is stable at 6 days 4°C.

Causes for Rejection: Samples will be rejected if the sample is improperly labeled

Use: High density lipoproteins (HDL) are responsible for the reverse transport of cholesterol from the peripheral cells to the liver. Here, cholesterol is transformed to bile acids which are excreted into the intestine via the biliary tract. Monitoring of HDL-cholesterol in serum is of clinical importance since an inverse correlation exists between serum HDL-cholesterol concentrations and the risk of atherosclerotic disease. Elevated HDL-cholesterol concentrations are protective against coronary heart disease, while reduced HDL-cholesterol concentrations, particularly in conjunction with elevated triglycerides, increase the cardiovascular risk.

Methodology: Enzymatic Photometric

Goal Value:
Male:  ≥40 mg/dL
Female:  ≥50 mg/dL
 
Note: If a gender is not provided, the result will default to the male goal value.
 
Additional Information: HDL cholesterol levels are temporarily low during acute illness, immediately following a heart attack, or during stress (like from surgery or an accident). Following illness, HDL cholesterol levels should be measured after at least six weeks. HDL cholesterol levels may also change during pregnancy, and therefore levels should be measured after at least six weeks following birth.
 

Triglycerides

CPT Code: 84478

Order Code: C119

Synonyms: Trigs, TGs

Sample Type: Serum

Minimum Volume: 0.5 ml

Tube Type: Tiger top tube

Patient Preparation: Patient must be fasting 12 hours prior to being drawn

Collection:
1. Draw sample and gently invert 5 times (DO NOT SHAKE)
2. Let the blood clot for 30 minutes
3. Centrifuge sample at 1300 rcf for 30 minutes

Storage Instructions: Cleveland HeartLab recommends that samples be stored at 4°C and shipped same day as draw. The sample is stable for 7 days at 4°C

Causes of Rejection:
Samples will be rejected if the sample is improperly labeled

Use: Triglycerides can be measured to evaluate hypo- or hyperlipidemia

Methodology:
Enzymatic Photometric

Goal Value:  <150 mg/dL

Additional Information: Patient should be fasting a minimum of 12 hours prior to specimen collection. Contraindications: certain drugs, endocrine imbalance, unstable weight, unbalanced or eccentric dietary eating habits may affect results.