Cleveland HeartLab A, Test

CPT Code: 82150
Order Code: C127
ABN Requirement:  No
Synonyms:  AML; Alpha-Amylase; Serum amylase
Specimen: Serum
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container:  Gel-barrier tube (SST, Tiger Top)


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport:  Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 1 month
Frozen (-20°C): Not Applicable
Deep Frozen (-70°C): Not Applicable

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; grossly lipemic and highly turbid samples

Methodology: Enzymatic Colorimetric Assay

Turn Around Time:  2 to 3 days

Reference Range:

Age U/L
All Ages 28-100

Urgent Values:

Age Urgent Value U/L
All Ages ≥300

Clinical Significance: The major source of amylase are the pancreas and salivary glands. The most common cause of elevation of serum amylase is inflammation of the pancreas (pancreatitis). In acute pancreatitis, serum amylase begins to rise within 6-24 hours, remains elevated for a few days and returns to normal in 3-7 days. Other causes of elevated serum amylase are inflammation of salivary glands (mumps), biliary tract disease, and bowel obstruction. Elevated serum amylase can also be seem with drugs (e.g., morphine) which constrict the pancreatic duct sphincter preventing excretion of amylase into the intestine.

Limitations: Icodextrin-based drugs may lead to decreased amylase results. In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.