ANA by IFA with Reflex

Cleveland HeartLab A, Test

NEW YORK DOH APPROVED: NO
CPT Code: 86038
Order Code: C2500
ABN Requirement: No
Synonyms: Antinuclear Antibody; Antinuclear Factor; ANAF
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Special Information: If ANA screen by IFA is positive, ANA titer and pattern, DNA, SSA Ab, SSB Ab, Smith Ab, JO-1 Ab, Chromatin, Scleroderma, RNP Ab, Ribosomal RNP, and Centromere will be performed and billed.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 24 hours
Refrigerated (2-8°C):
7 days
Frozen (-20°C):
30 days
Deep Frozen (-70°C):
30 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Indirect Immunofluorescence Assay (IFA)

Turn Around Time: 2-5 days

Reference Range:

Test Age Normal Range
ANA All Ages Negative
ANA Titer All Ages Negative
ANA Pattern All Ages Negative

Intended Use: Screening test for systemic autoimmune disease.

Clinical Significance: Antinuclear antibodies are a group of autoantibodies produced by the immune system when it fails to adequately distinguish between “self” and “nonself.” ANA target substances found in the nucleus of a cell and cause organ and tissue damage.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.