Order Code: C2500
ABN Requirement: No
Synonyms: Antinuclear Antibody; Antinuclear Factor; ANAF
Volume: 1.0 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Let tube stand in a vertical position to allow blood to clot 30 minutes.
- Centrifuge at 1300 rcf for 10 minutes at room temperature.
Special Information: If ANA screen by IFA is positive, ANA titer and pattern, DNA, SSA Ab, SSB Ab, Smith Ab, JO-1 Ab, Chromatin, Scleroderma, RNP Ab, Ribosomal RNP, and Centromere will be performed and billed.
Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Ambient (15-25°C): 24 hours
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 30 days
Deep Frozen (-70°C): 30 days
Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits
Methodology: Indirect Immunofluorescence Assay (IFA)
Turn Around Time: 2-5 days
|ANA Titer||All Ages||Negative|
|ANA Pattern||All Ages||Negative|
Intended Use: Screening test for systemic autoimmune disease.
Clinical Significance: Antinuclear antibodies are a group of autoantibodies produced by the immune system when it fails to adequately distinguish between “self” and “nonself.” ANA target substances found in the nucleus of a cell and cause organ and tissue damage.