ANA by IFA with Reflex

Cleveland HeartLab A, Test

NEW YORK DOH APPROVED: NO
CPT Code: 86038
Order Code: C2500
ABN Requirement: No
Synonyms: Antinuclear Antibody; Antinuclear Factor; ANAF
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes at room temperature.

Special Information: If ANA screen by IFA is positive, ANA titer and pattern, DNA, SSA Ab, SSB Ab, Smith Ab, JO-1 Ab, Chromatin, Scleroderma, RNP Ab, Ribosomal RNP, and Centromere will be performed and billed.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

Stability:

Ambient (15-25°C): 24 hours
Refrigerated (2-8°C):
7 days
Frozen (-20°C):
30 days
Deep Frozen (-70°C):
30 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Indirect Immunofluorescence Assay (IFA)

Turn Around Time: 2-5 days

Reference Range:

Test Age Normal Range
ANA All Ages Negative
ANA Titer All Ages Negative
ANA Pattern All Ages Negative

Intended Use: Screening test for systemic autoimmune disease.

Clinical Significance: Antinuclear antibodies are a group of autoantibodies produced by the immune system when it fails to adequately distinguish between “self” and “nonself.” ANA target substances found in the nucleus of a cell and cause organ and tissue damage.