Creatine Kinase-MB (CKMB)

Cleveland HeartLab C, Test

CPT Code: 82553
Order Code: C2138
ABN Requirement:  No
Synonyms: CK MB; CPK MB; Creatine Kinase MB Isoenzyme
Specimen: Serum
Volume: 1.0 mL
Minimum Volume: 0.5mL
Container:  Gel-barrier tube (SST, Tiger Top)


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 8-10 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into a labeled transport tube and cap tightly.

Special Information: Samples should not be taken from patients receiving therapy with high biotin doses (i.e., >5 mg/day) until at least 8 hours following the most recent biotin administration.

Transport: Store aliquoted serum at -20°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

  • Please note: Ship frozen serum on dry ice.


Ambient (15-25°C): 5 hours
Refrigerated (2-8°C): 12 hours
Frozen (-20°C): 3 months
Deep Frozen (-70°C): 3 months

Causes for Rejection: improper labeling; samples not stored properly; samples older than stability limits

Methodology: Electrochemiluminescence Immunoassay (ECLIA)

Turn Around Time:  2 – 3 days

Reference Range:

Male, All Ages <7.7
Female, All Ages <4.3

Use: A creatine kinase-MB test can be used in individuals with chest pain to distinguish between a myocardial infarction and other causes, and to monitor the efficacy of anti-thrombotic therapy in individuals who are experiencing a myocardial infarction.

Limitations: In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.