Order Code: 1342
ABN Requirement: No
Volume: 0.5 mL
Minimum Volume: 0.3 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge for 10 minutes.
Special Instructions: Serum sample must be stored in refrigerated temperature within an hour of collection.
Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.
Ambient (15-25°C): not acceptable
Refrigerated (2-8°C): 5 days
Frozen (-20°C): 7 days
Deep frozen (-70°C): 21 days
Note: When possible, we encourage samples to be shipped the same day as drawn in order to maintain sample integrity.
Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; samples that are grossly hemolyzed, lipemic, or icteric
Methodology: Microfluidics Electrophoresis
Turn Around Time: 4 – 7 days
HDL2b may be used for individuals at risk of diabetes or cardiovascular disease, those with cardiovascular disease or those with low total HDL levels or high triglyceride levels. The relative risk ranges are as follows: relatively low risk F >28%, M >26%; relatively moderate risk F 18-28%, M 18-26%; relatively high risk M/F <18%.
Use: The HDL2b test may be used for individuals at risk of diabetes or cardiovascular disease, those with cardiovascular disease or those with low total HDL levels or high triglyceride levels.
- Elevated total cholesterol and low HDL cholesterol levels, as well as high triglyceride levels, are associated with low HDL2b levels1-4.
- Reduced HDL2b levels have been associated with insulin resistance5.
- Women tend to have higher levels of HDL2b than men, and HDL2b levels tend to decrease as a person’s BMI increases6.
- HDL2b levels may be significantly increased by a combination of caloric restriction and high-intensity exercise7.
- Johansson J et al. High density lipoproteins and coronary atherosclerosis. A strong inverse relation with the largest particles is confined to normotriglyceridemic patients. Arterioscler Thromb. 1991; 11:174-182.
- Yang Y et al. Relationship between plasma lipid concentrations and HDL subclasses. Clin Chim Acta. 2005; 354: 49-58.
- Tian L et al. Characteristics of high-density lipoprotein subclasses distribution for subjects with desirable total cholesterol levels. Lipids in Health and Disease. 2011; 10: 64-72.
- Jia L et al. Alterations of high-density lipoprotein subclasses in hypercholesterolemia and combined hyperlipidemia. Int J Cardiol. 2007; 120: 331-337.
- Tilly-Kiesi M et al. Hyperinsulinemia and insulin resistance are associated with multiple abnormalities of lipoprotein subclasses in glucose-tolerant relatives of NIDDM patients. J Lipid Res. 1996; 37:1569-1578.
- Williams PT et al. Associations of age, adiposity, alcohol intake, menstrual status, and estrogen therapy with high-density lipoprotein subclasses. Arterioscler Thromb. 1993; 13: 1654-1661.
- Williams PT et al. Effects of low-fat diet, calorie restriction, and running on lipoprotein subfraction concentrations in moderately overweight men. Metabolism. 1994; 43: 655-663.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.