Order Code: C121
ABN Requirement: No
Synonyms: High Sensitivity CRP; Ultra-sensitive CRP; hsCRP
Specimen: Serum (preferred); EDTA Plasma
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Serum: Gel-barrier tube (SST, Tiger Top)
Plasma: EDTA (Lavender Top tube)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge at 1300 rcf for 10 minutes.
- Draw and gently invert 8 to 10 times.
- Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
- Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
Note: This ensures that the buffy coat and red cells remain undisturbed.
- Aliquot plasma into labeled transport tube labeled as “EDTA plasma” and cap tightly. Discard original tube.
- Store transport tube refrigerated at 2-8°C until ready to ship.
Transport: Store serum or EDTA plasma at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Ambient (15-25°C): 11 days
Refrigerated (2-8°C): 2 months
Frozen (-20°C): 3 years
Deep Frozen (-70°C): 3 years
Causes for Rejection: Specimens other than serum or EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits
Turn Around Time: 1 to 3 days
Intended Use: The hsCRP test is a useful tool to determine cardiovascular disease risk in individuals at intermediate risk (10-year risk of 10-20%) of developing CHD who are metabolically stable without inflammatory or infectious conditions.
Limitations: In very rare cases gammopathy, particularly type IgM, may cause unreliable results. Although measures were taken to minimize interference caused by human anti-mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes.