High-sensitivity CRP (hsCRP)

Cleveland HeartLab H, Inflammation, Test

NEW YORK DOH APPROVED: YES
CPT Code: 86141
Order Code: C121
ABN Requirement:  No
Synonyms: High Sensitivity CRP; Ultra-sensitive CRP; hsCRP
Specimen: Serum (preferred); EDTA Plasma
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container:

Serum: Gel-barrier tube (SST, Tiger Top)

Plasma: EDTA (Lavender Top tube)

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes.

EDTA Plasma:

  1. Draw and gently invert 8 to 10 times.
  2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
  3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma” and cap tightly. Discard original tube.
  5. Store transport tube refrigerated at 2-8°C until ready to ship.

Transport: Store serum or EDTA plasma at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

Stability:

Ambient (15-25°C): 11 days
Refrigerated (2-8°C): 2 months
Frozen (-20°C): 3 years
Deep Frozen (-70°C): 3 years

Causes for Rejection: Specimens other than serum or EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Immunoturbidimetric

Turn Around Time: 1 to 3 days

Relative Risk:

Age Low Risk
mg/L
Moderate Risk
mg/L
High Risk
mg/L
All Ages <1.0 1.0-3.0 >3.0

Intended Use: The hsCRP test is a useful tool to determine cardiovascular disease risk in individuals at intermediate risk (10-year risk of 10-20%) of developing CHD who are metabolically stable without inflammatory or infectious conditions.

Limitations: In very rare cases gammopathy, particularly type IgM, may cause unreliable results. Although measures were taken to minimize interference caused by human anti-mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes.