Insulin Like Growth Factor 1 (IGF-1)

Cleveland HeartLab I, Test

NEW YORK DOH APPROVED: YES
CPT Code: 84305
Order Code: C365
ABN Requirement: No
Synonyms: Somatomedin C; IGF 1; ILGF1
Specimen: Serum or EDTA Plasma
Volume:  0.5 mL
Minimum Volume:  0.3 mL
Container: Gel-barrier tube (SST, Tiger Top) or EDTA (Lavender Top) tube

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

EDTA Plasma:

  1. Draw and gently invert 8 to 10 times.
  2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
  3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  4. Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
  5. Store transport tube refrigerated at 2-8°C until ready to ship.

Special Information: The supplementation of high biotin levels, exceeding the recommended daily allowance of 0.03 mg, could lead to incorrect blood test results with immunoassays that are based on biotin-streptavidin interactions. Clinicians should consider biotin interference as a source of error, when clinically suspicious of the laboratory result.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 3 days
Refrigerated (2-8°C):
3 days
Frozen (-20°C):
1 year
Deep Frozen (-70°C):
1 year

Causes for Rejection: Specimens other than serum or EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Chemiluminescence Assay

Turn Around Time: 1 to 5 days

Reference Range:

Age (years) Male (ng/mL) Female (ng/mL)
<1 27-157 18-126
 1 30-167 20-132
 2 34-184 22-145
 3 39-205 26-164
 4 44-225 31-188
 5 50-246 36-214
 6 56-267 42-240
 7 63-292 49-270
 8 72-323 57-305
 9 84-362 67-349
 10 97-407 80-400
 11 112-454 93-453
 12 126-499 105-499
13 139-533 116-533
14 148-551 123-552
15 152-554 127-554
16 153-542 128-542
17 151-521 125-517
18 146-494 121-486
19 140-463 114-451
20 133-430 108-416
21-25 115-355 93-342
26-30 98-282 78-270
31-35 88-246 73-243
36-40 83-233 69-227
41-45 75-216 62-204
46-50 67-205 57-195
51-56 61-200 53-190
57-60 54-194 46-172
61-65 49-188 42-169
66-70 47-192 38-163
71-75 41-179 37-165
76-80 37-172 35-165
81-85 34-165 34-172
86-90 32-166 34-178
Tanner Stage I 81-255 86-323
Tanner Stage II 106-432 118-451
Tanner Stage III 245-511 258-528
Tanner Stage IV 223-578 224-586
Tanner Stage V 227-518 188-512

 

Intended Use: For diagnosis of hypopituitarism and acromegaly.

Clinical Significance: Low IGF-1 is observed in GH deficiency or GH resistance. Elevated IGF-1 indicate a sustained overproduction of GH, or excessive rhGH therapy.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.