Myeloperoxidase (MPO)

Cleveland HeartLab Inflammation, M, Test

NEW YORK DOH APPROVED: YES
CPT Code: 83876
Order Code: C133
ABN Requirement:  No
Synonyms: MPO
Specimen: EDTA Plasma
Volume: 1.0 mL
Minimum Volume: 0.5 mL
Container:  EDTA (Lavender Top tube)

Collection:

Plasma:

    1. Draw and gently invert 8 to 10 times.
    2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
    3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
      Note: This ensures that the buffy coat and red cells remain undisturbed.
    4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma” and cap tightly. Discard original tube.
    5. Store transport tube refrigerated at 2-8oC until ready to ship.

Transport:  Store plasma at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

Stability:

Ambient (15-25°C): not acceptable
Refrigerated (2-8°C): 8 days
Frozen (-20°C): 6 months
Deep Frozen (-70°C): 6 months

Causes for Rejection: Grossly hemolyzed samples; specimens other than EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Turbidimetric Immunoassay (TIA)

Turn Around Time:  1 to 3 days

Relative Risk:

Age Low Risk
pmol/L
Moderate Risk
pmol/L
High Risk
pmol/L
All Ages <470 470 – 539 ≥540

 

Use: Myeloperoxidase testing may be used for individuals with multiple risk factors for cardiovascular disease, or those with established disease.

Limitations:  Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.