PULS Cardiac Test™

Cleveland HeartLab New Tests, P, Test

CPT Code: 83520 (x7), 83036, 83718
Order Code: C1003
ABN Requirement:  No
Specimen: Serum and EDTA Whole Blood
Volume: 4.0 mL Serum and 2.5 mL Whole Blood
Minimum Volume: 1.0 mL Serum and 0.5 mL Whole Blood
Container:  Gel-barrier tube (SST, Tiger Top Tube) and EDTA (Lavender Top tube)



  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Whole Blood:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
  3. Do not centrifuge.

Special Instructions: A separate EDTA whole blood tube is needed for the PULS test. Collect additional samples for other testing that requires EDTA whole blood.

Please include all required patient information on requisition to report the PULS test:  

  • Height (feet and inches)
  • Weight (lbs)
  • BMI
  • Smoker (last 30 days) (Yes/No)
  • Diabetic (Type I or II) (Yes/No)
  • Hypertension (≥140/90 mmHg) (Yes/No)
  • Antihypertensive Rx (Yes/No)
  • Family History MI (Yes/No)
  • Patient History MI (Yes/No)
  • Excessive Stress (Yes/No)
  • Lipid Lowering Rx (Yes/No)
  • Low HDL (Yes/No)
  • Blood Pressure (Systolic/Diastolic) *At a minimum, the PULS test requires: Age, Gender, Family history of heart attack (parent, sibling, or child), Diagnosed Diabetes (Type I or II). If Cleveland HeartLab does not have this information, a PULS test cannot be resulted.

Please use PULS Cardiac Test requisition (RLF-0217) to order the PULS test. For PULS test requisition forms, please contact Cleveland HeartLab Customer Support at 1.866.358.9828, option 1.

Transport:  Store serum and EDTA whole blood at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.



Ambient (15-25°C): Not Acceptable
Refrigerated (2-8°C): 72 hours
Frozen (-20°C): 3 weeks
Deep Frozen (-70°C): 1 year

EDTA Whole Blood:

Ambient (15-25°C): 8 hours
Refrigerated (2-8°C): 1 week
Frozen (-20°C): 3 months
Deep Frozen (-70°C): 18 months

Causes for Rejection: Specimen identification issues; suboptimal specimen(s); improper specimen collection/handling/transport

Methodology: Chemiluminescent Immunoassay and Photometry

Turn Around Time:  10 to 12 days

Relative Risk:

Normal % Borderline % Elevated %
<3.50 3.50-7.49 ≥7.50

Clinical Significance: Cardiac risk assessments are used to identify the likelihood of a future cardiac event. The PULS Cardiac Test enhances risk assessment by evaluating clinical risk factors as well as 7 unique protein biomarkers associated with atherosclerotic disease progression and plaque vulnerability. PULS has demonstrated accuracy in predicting risk for cardiac events and was validated in the Multi-Ethnic Study of Atherosclerosis (MESA).1

1Cross DS et al. Curr Med Res Opin. 2012; 28: 1819-1830.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.