PULS Cardiac Test™

Cleveland HeartLab New Tests, P, Test

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CPT Code: 83520 (x7), 83036, 83718
Order Code: C1003
ABN Requirement:  No
Specimen: Serum and EDTA Whole Blood
Volume: 4.0 mL Serum and 2.5 mL Whole Blood
Minimum Volume: 1.0 mL Serum and 0.5 mL Whole Blood
Container:  Gel-barrier tube (SST, Tiger Top Tube) and EDTA (Lavender Top tube)

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes.

Whole Blood:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
  3. Do not centrifuge.

Special Instructions: A separate EDTA whole blood tube is needed for the PULS test. Collect additional samples for other testing that requires EDTA whole blood.

Transport:  Store serum and EDTA whole blood at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

Stability:

Serum:

Ambient (15-25°C): Not Acceptable
Refrigerated (2-8°C): 72 hours
Frozen (-20°C): 3 weeks
Deep Frozen (-70°C): 1 year

EDTA Whole Blood:

Ambient (15-25°C): 8 hours
Refrigerated (2-8°C): 1 week
Frozen (-20°C): 3 months
Deep Frozen (-70°C): 18 months

Causes for Rejection: Specimen identification issues; suboptimal specimen(s); improper specimen collection/handling/transport

Methodology: Chemiluminescent Immunoassay and Photometry

Turn Around Time:  10 to 12 days

Relative Risk:

Normal % Borderline % Elevated %
<3.50 3.50-7.49 ≥7.50

Clinical Significance: Cardiac risk assessments are used to identify the likelihood of a future cardiac event. The PULS Cardiac Test enhances risk assessment by evaluating clinical risk factors as well as 7 unique protein biomarkers associated with atherosclerotic disease progression and plaque vulnerability. PULS has demonstrated accuracy in predicting risk for cardiac events and was validated in the Multi-Ethnic Study of Atherosclerosis (MESA).1

1Cross DS et al. Curr Med Res Opin. 2012; 28: 1819-1830.