RBC Folate

CPT Code: 82747
Order Code: C1259
ABN Requirement: No
Synonym: Folic Acid, Red Cell Folate
Specimen: EDTA Whole Blood
Volume: 2.0 mL
Minimum Volume: 0.3 mL
Container: EDTA (Lavender Top) tube


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 8-10 times immediately after draw. DO NOT SHAKE.
  3. Do not centrifuge.

Special Instructions: Collect additional EDTA lavender top tube for other testing that requires EDTA whole blood. Folate is light sensitive. It is recommended that exposure to light during handling and storage be minimized.

Transport: Store EDTA whole blood at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 72 hours
Refrigerated (2-8°C): 72 hours
Frozen (-20°C): 1 month
Deep Frozen (-70°C): 1 month

Note: Hematocrit is only stable for 48 hours at 2-8°C.

Causes for Rejection: Specimens other than EDTA whole blood; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Immunoassay (IA)

Turn Around Time: 2-3 days

Reference Range:

Age ng/mL
All Ages >280

Please Note: When a hematocrit (HCT) value cannot be obtained, a default HCT value will be used.

Clinical Significance: Folate levels have diagnostic significance in nutritional deficiencies, especially in cases of severe alcoholism, function damage to the upper third of small bowel, pregnancy, and various forms of megaloblastic anemia. Since serum folate levels are subject to rapid changes reflecting diet and absorption, RBC folate may be a better diagnostic tool since the levels remain fairly constant.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.