Testosterone, Free, Bioavailable, and Total (MS) (Males <18 Years Old and Females)

Cleveland HeartLab T, Test

NEW YORK DOH APPROVED: NO

CPT Code: 82040 (x1), 84270 (x1), 84403 (x1)
Order Code: 1043
Includes: Testosterone, Total, MS; Testosterone, Free; Testosterone, Bioavailable; Sex Hormone Binding Globulin (SHBG); Albumin
ABN Requirement: No
Specimen: Serum
Volume:  3.0 mL
Minimum Volume:  1.5 mL
Container: Red Top (no gel barrier) tube

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into a labeled transport tube and cap tightly.

*Special Information: Please specify age and sex on the test requisition form.*

Transport: Store aliquoted serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 21 days
Frozen (-20°C): 60 days
Deep Frozen (-70°C): 60 days

Causes for Rejection: Specimens other than red top serum; improper labeling; samples not stored properly; samples older than stability limits; grossly hemolyzed or lipemic specimens

Methodology: Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS), Spectrophotometry (SP), Immunochemiluminescent Assay, Calculation (CALC)

Turn Around Time: 4 to 5 days

Reference Range:

Testosterone, Total:

  Male (ng/dL) Female (ng/dL)
Cord Blood Not Established Not Established
1-10 days ≤187 ≤24
1-2 months 72-344 ≤17
3-4 months ≤201 ≤12
5-6 months ≤59 ≤13
7-11 months ≤16 ≤11
1-5 years ≤5 ≤8
6-7 years ≤25 ≤20
8-10 years ≤42 ≤35
11-12 years ≤260 ≤40
12-13 years ≤420 ≤40
14-17 years ≤1000 ≤40
≥18 years 250-1100 2-45

 

Testosterone, Free:

  Male (pg/mL) Female (pg/mL)
<1 year Not Established Not Established
1-11 years ≤1.3 ≤1.5
12-13 years ≤64.0 ≤1.5
14-17 years 4.0-100.0 ≤3.6
18-69 years 46.0-224.0 0.2-5.0
70-89 years 6.0-73.0 0.3-5.0

 

Testosterone, Bioavailable:

  Male (pg/mL) Female (pg/mL)
<1 year Primary data not available Primary data not available
1-11 years ≤5.4 ≤3.4
12-13 years ≤140.0 ≤3.4
14-17 years 8.0-210.0 ≤7.8
18-69 years 110.0-575.0 0.5-8.5
70-89 years 15.0-150.0 0.5-8.8

 

Sex Hormone Binding Globulin (SHBG):

  Male (nmol/L) Female (nmol/L)
Infant-2 years Not Established Not Established
3-9 years 32-158 32-158
10-13 years 20-166 24-120
14-17 years 20-87 12-150
18-55 years 10-50 17-124
≥56 years 22-77 14-73

 

Albumin:

  Male (g/dL) Female (g/dL)
All Ages 3.6-5.1 3.6-5.1

Clinical Significance: Helpful in assessing testicular function in prepubescent, hypogonadal males, and in managing hirsutism and virilization in females.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.