Testosterone, Free (Dialysis) and Total (MS) (Males <18 Years Old and Females)

Cleveland HeartLab T, Test

NEW YORK DOH APPROVED: NO

CPT Code: 84402, 84403
Order Code: 1300
Includes: Testosterone, Total, (LC/MS/MS) and Testosterone, Free (Dialysis)
ABN Requirement: No
Synonyms: Free Testosterone
Specimen: Serum
Volume:  0.9 mL
Minimum Volume:  0.4 mL
Container:  Red Top (no gel barrier) tube

Collection:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into a labeled transport tube and cap tightly.

*Special Information: Please specify age and sex on the test requisition form.*

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 21 days
Frozen (-20°C): 2 years
Deep Frozen (-70°C): 2 years

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia

Methodology: Chromatography/Mass Spectrometry, Equilibrium Dialysis, Calculation

Turn Around Time: 5 to 6 days

Reference Range:

Testosterone, Free:

  Male Female
5-9 years ≤5.3 pg/mL 0.2-5.0 pg/mL
10-13 years 0.7-52.0 pg/mL 0.1-7.4 pg/mL
14-17 years 18.0-111.0 pg/mL 0.5-3.9 pg/mL
18-69 years 35.0-155.0 pg/mL 0.1-6.4 pg/ml
70-89 years 30.0-135.0 0.2-3.7 pg/mL
Tanner Stage I ≤5 ng/dL ≤8 ng/dL
Tanner Stage II ≤167 ng/dL ≤24 ng/dL
Tanner Stage III 21-719 ng/dL ≤28 ng/dL
Tanner Stage IV 25-912 ng/dL ≤31 ng/dL
Tanner Stage V 110-975 ng/dL ≤33 ng/dL

 

Testosterone, Total:

  Male (ng/dL) Female (ng/dL)
Cord Blood 17-61 16-44
1-2 months 72-344 ≤17
3-4 months ≤201 ≤12
5-6 months ≤59 ≤13
7-11 months ≤16 ≤11
1-5 years ≤5 ≤8
6-7 years ≤25 ≤20
8-10 years ≤42 ≤35
11 years ≤260 ≤40
12-13 years ≤420 ≤40
14-17 years ≤1000 ≤40
≥18 years 250-1100 2-45

Clinical Significance: Helpful in assessing testicular function in males, and in managing hirsutism and virilization in females.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.