Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum
CPT Code: 84155, 84165
Order Code: 1212
Includes: Total Protein, Albumin, Alpha-1 Globulin, Alpha-2 Globulin, Beta-1 Globulin, Beta-2 Globulin, Gamma Globulin, Abnormal Protein Band 1 (if present), Abnormal Protein Band 2 (if present), Abnormal Protein Band 3 (if present), Immunofixation (IFE) (if reflexed)
ABN Requirement: No
Volume: 4.0 mL
Minimum Volume: 0.5 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge for 10 minutes.
Special Information: If abnormal banding is detected, Immunofixation (IFE) will be performed at an additional charge (CPT code 86334).
Fasting: Overnight fasting is preferred
Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.
Ambient (15-25°C): 4 days
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 28 days
Causes for Rejection: Specimens other than serum; grossly hemolyzed specimens; grossly lipemic specimens; improper labeling; samples not stored properly; samples older than stability limits
Methodology: Capillary Zone Electrophoresis (CZE), Spectrophotometry (SP)
Turn Around Time: 2 to 3 days
Protein, Total, Serum:
Clinical Significance: Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.