Cleveland HeartLab A, Metabolic, New Tests, Test

CPT Code: 83516
Order Code: C314
ABN Requirement: No
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.3 mL
Container: Gel-barrier tube (SST, Tiger Top)


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.


Ambient (15-25°C): 3 days
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 21 days
Deep frozen (-70°C): Not Acceptable

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Enzyme Linked Immunosorbent Assay (ELISA)

Turn Around Time: 7 to 10 days

Reference Range:

BMI Adiponectin (µg/mL)
Male Female
<25 kg/m2 4-26 5-37
25-30 kg/m2 4-20 5-28
>30 kg/m2 2-20 4-22

Intended Use: The adiponectin test may be performed on individuals at risk of metabolic syndrome or diabetes due to poor lifestyle choices.

Clinical Significance:

  • Individuals with low adiponectin levels have a 3X greater risk of developing metabolic syndrome1.
  • Men with two or more risk factors for metabolic syndrome and high adiponectin levels are half as likely to develop metabolic syndrome as men with low adiponectin levels2.
  • Individuals with low levels of adiponectin are up to 9X as likely to develop type 2 diabetes3.
  • Individuals with low adiponectin levels have a 2X increase in the prevalence of CAD4.
  • Adiponectin levels in the blood can be increased by thiazolidinediones, such as pioglitazone5.


  1. Chen SJ et al. PLoS ONE. 2012; 7: e45693.
  2. Kotooka N et al. Int J Cardiol. 2012 Nov 26. pii: S0167-5273(12)01441-6. doi:10.1016/j.ijcard.2012.10.066. [Epub ahead of print].
  3. Daimon M et al. Diabetes Care. 2003; 26: 2015-2020.
  4. Kumada M et al. Arterioscler Thromb Vasc Biol. 2003; 23: 35-39.
  5. McCoy RG et al. Mayo Clin Proc. 2012; 87: 561-570.