Alanine Amino Transferase (ALT)

Cleveland HeartLab A, Test

NEW YORK DOH APPROVED: YES
CPT Code: 84460
Order Code: C112
ABN Requirement:  No
Synonyms: ALT; Serum Glutamate Pyruvate Transaminase; SGPT
Specimen: Serum
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

Stability:

Ambient (15-25°C): 3 days
Refrigerated (2-8°C): 1 week
Frozen (-20°C): not acceptable
Deep Frozen (-70°C): >1 week

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; hemolyzed specimens

Methodology: Photometric

Turn Around Time: 1 to 3 days

Reference Range:

Age Male
U/L
Female
U/L
All Ages 5-50 0-45


Intended Use:
The alanine aminotransferase test may be ordered for individuals at risk for liver disease or damage, including those with diabetes or those on liver-damaging medications such as statins.

Limitations: Contamination with erythrocytes will elevate results. Calcium dobesilate and Isonizid can cause artificially low ALT results at therapeutic concentrations. Furosemide can cause artificially high ALT results at therapeutic concentrations. Cyanokit (Hydroxocobalamin) may cause interference with results. In very rare cases gammopathy, in particular type IgM (Waldenstrom’s macrogrlobulinemia), may cause unreliable results.