ANA Screen, IFA, with Reflex to Titer and Pattern

Cassandra Greaves A, Test

NEW YORK DOH APPROVED: YES

CPT Code: 86038
Order Code: 249
Includes: If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code: 86039).
Alternative Names:
FANA, Fluorescent ANA, Hep-2, Antinuclear Antibody Screen
ABN Requirement:
No
Specimen
: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.

Stability:

Ambient (15-25°C): 4 days
Refrigerated (2-8°C):
7 days
Frozen (-20°C):
30 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia; microbial contamination

Methodology: Immunofluorescence Assay (IFA)

Turn Around Time:  2 to 3 days

Reference Range:

ANA Screen Negative
ANA Titer
<1:40 Negative
1:40-1:80 Low antibody level
>1:80 Elevated antibody level

Clinical Significance: Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren’s syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.