Order Code: C1406
ABN Requirement: No
Specimen: EDTA Plasma or Red Top Serum
Volume: 1.0 mL
Minimum Volume: 0.3 mL
Container: EDTA (Lavender Top) tube or Red Top (no gel barrier) tube
- Draw and gently invert 8 to 10 times.
- Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
- Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
Note: This ensures that the buffy coat and red cells remain undisturbed.
- Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
- Store transport tube refrigerated at 2-8°C until ready to ship.
Special Information: Early morning specimen is preferred.
- Collect and label sample according to standard protocols.
- Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge at 1300 rcf for 10 minutes
- Aliquot serum into labeled transport tube and cap tightly.
Transport: Store EDTA plasma or serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Ambient (15-25°C): 14 days
Refrigerated (2-8°C): 28 days
Frozen (-20°C): 2 years
Deep Frozen (-70°C): 2 years
Causes for Rejection: Samples other than EDTA plasma or red top serum; serum from SST tubes; specimens received in glass tubes; improper labeling; samples not stored properly; samples older than stability limits
Methodology: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Turn Around Time: 9 days
|Premature (31-35 weeks)||≤480|
|Tanner Stages, Male||ng/dL|
|Tanner Stages, Female||ng/dL|
Intended Use: The androstenedione test is indicated for the evaluation of patients with androgen excess and for managing patients with Congenital Adrenal Hyperplasia.
Additional Information: An early morning specimen is preferred. Certain drugs, including levonorgestrel and corticotrophin can increase concentrations of androstenedione, while corticosteroids including dexamethasone can decrease androstenedione levels.