Cleveland HeartLab A, Test

CPT Code: 82157
Order Code: 1173
ABN Requirement:  No
Specimen: EDTA Plasma or Red Top Serum
Volume:  1.0 mL
Minimum Volume:  0.3 mL
Container: EDTA (Lavender Top) tube or Red Top (no gel barrier) tube


EDTA Plasma

  1. Draw and gently invert 8 to 10 times.
  2. Centrifuge for 10 minutes.
  3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  4. Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
  5. Store transport tube refrigerated at 2-8°C until ready to ship.

Special Information: Early morning specimen is preferred.


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into labeled transport tube and cap tightly.

Transport: Store EDTA plasma or serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 14 days
Refrigerated (2-8°C): 28 days
Frozen (-20°C): 2 years

Causes for Rejection: Samples other than EDTA plasma or red top serum; serum from SST tubes; specimens received in glass tubes; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Turn Around Time: 5 to 6 days

Reference Range:

Adult Male ng/dL
18-30 Years 50-220
31-50 Years 40-190
51-60 Years 50-220
Adult Female ng/dL
Follicular 35-250
Luteal 30-235
Postmenopausal 20-75
Pediatric ng/dL
1-12 Months 6-78
1-4 Years 5-51
5-9 Years 6-115
10-13 Years 12-221
14-17 Years 22-225
Infants ng/dL
Premature (31-35 weeks) ≤480
Term ≤290
Tanner Stages, Male ng/dL
II-III 17-82
IV-V 57-150
Tanner Stages, Female ng/dL
II-III 43-180
IV-V 73-220

Intended Use: The androstenedione test is indicated for the evaluation of patients with androgen excess and for managing patients with Congenital Adrenal Hyperplasia.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.