Order Code: C123
ABN Requirement: No
Synonyms: Apo B; Apo B100; Beta Apolipoprotein
Specimen: Serum or EDTA Plasma
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top) or EDTA (Lavender Top) tube
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge at 1300 rcf for 10 minutes.
- Draw and gently invert 8 to 10 times.
- Place specimen on ice after draw.
- Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
- Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
Note: This ensures that the buffy coat and red cells remain undisturbed.
- Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
- Store transport tube refrigerated at 2-8°C until ready to ship.
Patient Preparation: Patient must be fasting 12 to 14 hours
Transport: Store serum or EDTA plasma at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Ambient (15-25°C): 1 day
Refrigerated (2-8°C): 8 days
Frozen (-20°C): 2 months
Deep Frozen (-70°C): 2 months
Causes for Rejection: Specimens other than serum or EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits
Turn Around Time: 1 to 3 days
Use: An Apo B test may have utility for individuals undergoing management for lipoprotein abnormalities, individuals with established coronary heart disease or diabetes, and individuals with two or more major risk factors for coronary heart disease.
Limitations: In very rare cases gammopathy may cause unreliable results.