Cancer Antigen 125

Cleveland HeartLab C, Test

CPT Code: 86304
Order Code: C130
ABN Requirement:  No
Synonyms: CA 125; Carbohydrate Antigen 125
Specimen: Serum
Volume: 0.5 mL
Minimum Volume: 0.3 mL
Container: Gel-barrier tube (SST, Tiger Top)


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Special Instructions: Samples should not be taken from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 8 days
Refrigerated (2-8°C): 5 days
Frozen (-20°C): 24 months
Deep Frozen (-70°C): 24 months

Causes for rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Electrochemiluminescence Immunoassay (ECLIA)

Turn Around Time: 1 – 5 days

Reference Range:

Age U/mL
All Ages <36

Intended Use: 
CA-125 testing is primarily used to monitor ovarian cancer treatment. It is also used to detect the recurrence of cancer after termination of treatment.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.