Order Code: C1547
Includes: Tissue Transglutaminase Ab (IgG), Tissue Transglutaminase Ab (IgA), Gliadin (Deamidated Peptide) Ab (IgG, IgA), Immunoglobulin A (IgA)
ABN Requirement: No
Volume: 5.0 mL
Minimum Volume: 2.5 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge for 10 minutes.
Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.
Ambient (15-25°C): 72 hours
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 30 days
Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia
Turn Around Time: 5 to 6 days
|Tissue Transglutaminase, IgG||All Ages||
|Tissue Transglutaminase Ab, IgA||All Ages||<4 U/mL
No antibody detected
|Gliadin (Deamidated Peptide) Ab IgG||All Ages||<20 Units
Antibody not detected
|Gliadin (Deamidated Peptide) Ab IgA||All Ages||<20 Units
Antibody not detected
|IgA, Serum||Cord Blood||1-3 mg/dL|
|1-28 days||2-40 mg/dL|
|1-3 months||3-40 mg/dL|
|4-6 months||7-47 mg/dL|
|7-11 months||12-53 mg/dL|
|1 year||20-73 mg/dL|
|2 years||20-99 mg/dL|
|3-5 years||22-140 mg/dL|
|6-8 years||31-180 mg/dL|
|9-11 years||33-200 mg/dL|
|12-16 years||36-220 mg/dL|
|17-60 years||47-310 mg/dL|
|61 years or older||
Clinical Significance: Tissue Transglutaminase Antibody IgG and IgA are useful in diagnosing gluten-sensitive enteropathies, such as Celiac Sprue Disease, and an associated skin condition, dermatitis herpetiformis in patients who are IgG- or IgA-deficient.
Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus, gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamination of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.
Increased IgA is associated with monoclonal IgA myeloma, respiratory, and gastrointestinal infections, and malabsorption; decreased IgA is found in selective IgA deficiency and in ataxia telangiectasia.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.