Hepatitis B Surface Antigen

Cleveland HeartLab H, Test

NEW YORK DOH APPROVED: NO
CPT Code: 87340
Order Code: C2423
ABN Requirement: No
Synonyms: Hepatitis B Antigen; HBsAg
Specimen: Serum or EDTA Plasma
Volume:  1.0 mL
Minimum Volume:  0.8 mL
Container: Gel-barrier tube (SST, Tiger Top) or EDTA (Lavender Top) tube

Collection:

Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

EDTA Plasma:

  1. Draw and gently invert 8 to 10 times.
  2. Centrifuge for 10 minutes.
  3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  4. Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
  5. Store transport tube refrigerated at 2-8°C until ready to ship.

Note:Hepatitis B Surface Antigen Confirmation will be performed at an additional charge (CPT 87341) on all initially reactive Hepatitis B Surface Antigen tests.

Special Information: Not intended for use in screening blood, plasma, or tissue donors. Performance has not been established for the use of cadaveric specimens or the use of body fluids other than human serum.

Transport: Store serum or plasma at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 24 hours
Refrigerated (2-8°C):
6 days
Frozen (-20°C): 14 days
Deep Frozen (-70°C): 14 days

Causes for Rejection: Specimens other than serum or EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits; heat inactivated specimens; pooled specimens; grossly hemolyzed specimens; specimens with obvious microbial contamination

Methodology: Chemiluminescent Microparticle Immunoassay (CMIA)

Turn Around Time: 2-3 days

Reference Range:

AgeNormal Range
All AgesNegative

Clinical Information: Current methods for the detection of hepatitis B surface antigen may not detect all potentially infected individuals. A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B virus. A nonreactive test result in individuals with prior exposure to hepatitis B may be due to antigen levels below the detection limit of this assay or lack of antigen reactivity to the antibodies in this assay. For diagnostic purposes, results should be used in conjunction with patient history and other hepatitis markers for diagnosis of acute and chronic infection.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.