CPT Code: 87538
Order Code: 34977
ABN Requirement: No
Specimen: EDTA Whole Blood
Volume: 1.0 mL
Minimum Volume: 0.4 mL
Container: EDTA (Lavender Top) tube
- Collect and label sample according to standard protocols.
- Gently invert tube 8-10 times immediately after draw. DO NOT SHAKE.
- Do not centrifuge.
HIV Testing Pathway Algorithm:
Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.
Ambient (15-25°C): 6 days
Refrigerated (2-8°C): 6 days
Frozen (-20°C): Unacceptable
Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; hemolyzed specimens; frozen specimens; heparinized whole blood
Methodology: Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Turn Around Time: 3 to 4 days
|HIV-2 DNA/RNA, Qualitative||Not Detected|
Clinical Significance: Infection with Human Immunodeficiency Virus type 2 (HIV-2) is currently diagnosed by the presence of antibodies to HIV-2, the detection of specific HIV-2 antigens or the ability to culture HIV-2 from blood, fluid, or tissue of infected persons. The Polymerase Chain Reaction (PCR) technique, on the other hand, allows for the exponential enzymatic amplification of selected HIV-2 DNA sequences present in clinical specimens. The amplified fragments are then detected by hybridization to an HIV-2 specific labeled prove. As a result, the HIV-2 DNA PCR is a highly sensitive and specific method to detect the presence of HIV-2 proviral DNA in clinical specimens. The diagnosis of infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional clinical tests. A negative PCR result indicates the absence of HIV-2 proviral DNA at detectable levels in the sample tests and does not exclude diagnosis of disease.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.