ABN Requirement: No
Synonyms: Immunoreactive Insulin
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge for 10 minutes.
Patient Preparation: Patient should fast for at least 8 hours prior to being drawn.
Special Instructions: Samples should not be taken from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last dose.
Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.
Ambient (15-25°C): not acceptable
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 6 months
Frozen (-70°C): 6 months
Causes for Rejection: Samples which are heat-inactivated; specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; hemolyzed specimens
Methodology: Electrochemiluminescence Immunoassay (ECLIA)
Turn Around Time: 1 to 3 days
|All Ages||1.0 – 24.0|
Use: Insulin testing is ordered to help evaluate insulin production, to monitor insulin resistance (particularly in obese individuals and women with polycystic ovary syndrome), to help determine the cause of hypoglycemia and to detect an insulin-producing tumor.
Limitations: Samples from patients treated with bovine, porcine or human insulin can sometimes contain anti-insulin antibodies which can affect the results. In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.