Iron

Cleveland HeartLab Anemia/Iron Metabolism, I, Test

NEW YORK DOH APPROVED: YES
CPT Code: 83540
Order Code: C147
ABN Requirement:  No
Synonyms: Fe; Fe²+
Specimen: Serum
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 21 days
Frozen (-20°C): >1 year
Deep Frozen (-70°C): >1 year

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; hemolyzed specimens

Methodology: Colorimetric Assay

Turn Around Time: 1 to 5 days

Reference Range:

Age μg/dL
All Ages 30-140


Urgent Values:

Age Urgent Value μg/dL
≤12 years ≥500

Intended Use: An iron test may be ordered to evaluate iron status. In addition, an iron test may be used to identify the cause of anemia, assess severity and monitor treatment. It may also be used to diagnose chronic gastrointestinal bleeding or blood disorders, including thalassemia and hemochromatosis.

Limitations: In patients treated with iron supplements or metal-binding drugs, the drug-bound iron may not react properly in the test, resulting in artificially low values. In the presence of high ferritin concentrations >1200 ug/L the assumption that serum iron is almost completely bound to transferrin is not valid anymore. Therefore, such iron results should not be used to calculate total Iron Binding Capacity (TIBC) or percent transferrin saturation (%SAT). Hemolysis may adversely affect results. In very rare cases, gammopathy, in particular type IgM (Waldenstrom’s macroglobulinemia), may cause unreliable results.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.