Lyme Disease Antibodies with Reflex to Blot (IgG, IgM)

Cleveland HeartLab L, Test

NEW YORK DOH APPROVED: YES
CPT Code: 86618
Order Code: C1473
ABN Requirement: No
Synonyms: Borrelia burgdorferi
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: Gel-barrier tube (SST, Tiger Top)

Collection:
Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Special Information: If Lyme Disease Antibody Screen is ≥0.90, then the Lyme Disease Antibodies (IgG, IgM) Immunoblot will be performed at an additional charge (CPT 86617 x2).

Stability:

Ambient (15-25°C): 7 days
Refrigerated (2-8°C):
14 days
Frozen (-20°C):
30 days
Deep Frozen (-70°C):
30 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; grossly hemolyzed, grossly lipemic, or grossly icteric samples; samples containing particulate matter; microbially contaminated serum

Methodology: Immunoassay (IA); Reflex: Immunoblot (IB)

Turn Around Time: 3-6 days

Reference Range:

Age Reference Range
All Ages Negative: ≤0.90
All Ages Equivocal: 0.91-1.09
All Ages Positive: ≥1.10


Clinical Significance:
Lyme disease is caused by a bacterium borrelia burgdorferi and is transmitted by ticks. EIA is the screening test with high sensitivity for antibody detection. Immunoblot testing qualitatively examines with high specificity antibodies in a patient’s specimen. Immunoblot testing is appropriate for confirming a detected EIA test result.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.