Rapid Plasma Reagin Test (RPR)

Cleveland HeartLab R, Test

CPT Code:  86592
Order Code: C1328
ABN Requirement: No
Synonyms: RPR; Syphilis
Specimen:  Serum
Volume:  1.0 mL
Minimum Volume:  0.2 mL
Container:  Gel-barrier tube (SST, Tiger Top)



  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge at 1300 rcf for 10 minutes at room temperature.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.


Ambient (15-25°C): 4 days
Refrigerated (2-8°C): 7
Frozen (-20°C):
30 days
Deep Frozen (-70°C): 
30 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; grossly hemolyzed or hyperlipemic specimens

Methodology: Flocculation

Turn Around Time: 2 to 4 days

Reference Range:

Age Normal Range
All Ages Non-Reactive

Intended Use: This test is a non-treponemal screening test for syphilis. Monitoring of RPR is helpful in assessing effectiveness of therapy.

Limitations: False-positive results have been associated in patients with infections, pregnancy, autoimmune disease, old age, Gaucher disease, and malignancy.