Vitamin B6

Cleveland HeartLab Test, V

CPT Code: 84207
Order Code: C2493
ABN Requirement: No
Synonyms: Pyridoxine; Pyridoxal Phosphate
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: Red top (no gel barrier) tube



  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Place specimen on ice after draw.
  4. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  5. Centrifuge at 1300 rcf for 10 minutes at room temperature.
  6. Aliquot serum into a labeled transport tube and cap tightly.

Special Information: Collect specimen after an overnight fast. Place specimen on ice after draw. Separate serum from cells ASAP and freeze. Protect specimen from light.

Transport: Store aliquoted serum at -20°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.

  • Please note: Ship frozen, aliquoted red top serum on dry ice.


Ambient (15-25°C): Not Acceptable
Refrigerated (2-8°C):
4 hours
Frozen (-20°C):
60 days
Deep Frozen (-70°C):
60 days

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; samples received not frozen

Methodology: High Performance Liquid Chromatography (HPLC)

Turn Around Time: 3-6 days

Reference Range:

All Ages20.0-125.0

Clinical Information: The biologically active form of vitamin B, pyridoxal 5-phosphate, is measured in this assay. This assay does not distinguish between pyridoxine, pyridoxamine, and pyridoxal forms of the vitamin, and does not detect pyridoxic acid. Pyridoxal 5-phosphate measured in a specimen collected following an 8-hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.