1,5-Anhydroglucitol (1,5-AG), Intermediate Glycemic Control

Cleveland HeartLab #'s, Test

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NEW YORK DOH APPROVED: YES
CPT Code: 84378
Order Code: 10378
ABN Requirement: No
Synonyms: 1,5-anhydroglucitol
Specimen: Serum or EDTA Plasma
Volume: 1.0 mL
Minimum Volume: 0.5 mL
Containe: Gel-barrier (SST) tube or EDTA (Lavender-Top) tube

Collection:

SST Serum:

  1. Collect and label SST tube according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. Do not shake.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

OR

EDTA Plasma:

  1. Collect and label a separate EDTA tube according to standard protocols.
    Note: If not collecting SST tube, two EDTA tubes must be collected.
  2. Gently invert tube 8-10 times immediately after draw. Do not shake.
  3. Centrifuge for 10 minutes
  4. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  5. Aliquot plasma into transport tube labeled as ‘EDTA plasma’ and cap rightly.
  6. Discard original tube.

Transport: Store and transport sample at either refrigerated (2-8°C) or room temperature and ship the same day per packaging instructions included with the provided shipping box.

Stability:

Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 28 days

Causes for Rejection: Improper labeling; samples not stored properly; samples older than stability limits; sodium fluoride/oxalate (gray-top) tube; heparinized plasma; unspun serum separator tube (SST)

Methodology: Enzymatic

Turn Around Time: 2 to 5 days

Reference Range:

Age Male (mcg/mL) Female (mcg/mL)
≤1 year Not Established Not Established
2-17 years 15.0-38.0 11.2-35.7
≥18 years 7.3-36.6 7.5-28.4

Clinical Significance: Diazyme 1,5-anhydroglucitol (1,5-AG) Assay is an enzymatic method intended for the quantitative determination of 1,5-anhydroglucitol (1,5-AG) in serum or plasma. The 1,5-AG Assay is for the intermediate term (preceding 1-2 weeks) monitoring of glycemic control in people with diabetes.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.