Cleveland Heartlab A, Test

CPT Code: 82024
Order Code: C2367
ABN Requirement: No
Specimen: EDTA Plasma
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: EDTA (Lavender Top) tube


  1. Place EDTA tube on ice prior to collection.
  2. Draw and gently invert 8 to 10 times.
  3. Place specimen on ice after draw.
  4. Centrifuge for 10 minutes.
  5. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  6. Aliquot plasma into labeled transport tube and cap tightly. Discard original tube.
  7. Store transport tube frozen at -20°C until ready to ship.

Special Information: EDTA tubes must be iced prior to collection. Plasma must be separated from cells immediately. Plasma aliquot should be frozen immediately in a plastic tube.

Transport: Store plasma frozen after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

  • Please note: Ship frozen EDTA plasma on dry ice.


Ambient (15-25°C): Not Acceptable
Refrigerated (2-8°C): 
Not Acceptable
Frozen (-20°C):
30 days
Deep Frozen (-70°C):
30 days

Causes for Rejection: Specimens other than EDTA plasma; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Chemiluminescence Immunoassay (CLIA)

Turn Around Time: 2-5 days

Reference Range:

Age pg/mL
All Ages <47

Use: ACTH levels in the blood are measured to help detect, diagnose, and monitor conditions associated with excessive or deficient cortisol in the body.

Clinical Significance: Plasma ACTH determinations are useful in the differential diagnosis of pituitary Cushing’s disease, Addison’s disease, autonomous ACTH-producing pituitary tumors, hypopituitarism with ACTH deficiency, and ectopic ACTH syndromes.

Limitations: Hemolyzed samples may indicate mistreatment of a specimen before receipt by the laboratory; hence results should be interpreted with caution.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.