Aspartate Amino Transferase (AST)

Cleveland Heartlab A, Test

CPT Code: 84450
Order Code: C113
ABN Requirement:  No
Synonyms: AST; GOT; Serum Glutamic Oxaloacetic Transaminase; SGOT; Transaminases
Specimen: Serum
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 1 day
Refrigerated (2-8°C): 7 days
Frozen (-20°C): not acceptable
Deep Frozen (-70°C): not acceptable

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits; hemolyzed specimens

Methodology: Photometric

Turn Around Time: 1 to 3 days

Reference Range:

Age U/L
0-2 years 10-60
3-5 years 10-55
6-10 years 5-50
≥11 years 7-40

Intended Use: The aspartate aminotransferase test may be ordered for the evaluation of individuals suspected of liver damage or disease, to monitor treatment for liver disease, to identify the cause of jaundice, and to monitor individuals receiving therapies that are toxic to the liver.

Limitations: Sample contamination with erythrocytes will elevate results because the AST level in erythrocytes is higher than in normal sera. Isoniazid can cause artificially low, and Furosemide artificially high, ALT results at therapeutic concentrations. In very rare cases gammopathy may cause unreliable results. Lipemia may cause unreliable results.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.