Calcitriol 1,25-Dihydroxyvitamin D

Cleveland Heartlab C, Test


CPT Code: 82652
Order Code: C1572
Includes: Vitamin D 1,25 (OH)2, Total; Vitamin D3 1,25 (OH)2; Vitamin D2 1,25 (OH)2
ABN Requirement:
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: Red Top (no gel barrier) tube


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into a labeled transport tube and cap tightly.

Note: If sample is submitted with less than 1.1 mL and needs to be repeated, the sample will be cancelled with the comment “TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat.”

Transport: Store aliquoted serum at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.


Ambient (15-25°C): 28 days
Refrigerated (2-8°C):
28 days
Frozen (-20°C):
28 days

Causes for Rejection: Specimens other than serum separated from cells; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; lipemia; icteric specimens

Methodology: Chromatography/Mass Spectrometry

Turn Around Time:  6 days

Reference Range:

Vitamin D 1,25 (OH)2, Total
1-9 Years31-87 pg/mL
10-13 Years30-83 pg/mL
14-17 Years19-83 pg/mL
Adult18-72 pg/mL
Vitamin D3 1,25 (OH)2Not Available
Vitamin D2 1,25 (OH)2Not Available

Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for information purposes only).

Clinical Significance: This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;257:266-281).

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.