Cardiolipin Antibodies (IgA, IgG, IgM)

Cleveland Heartlab C, Test


CPT Code: 86147 (x3)
Order Code: C1469
Tests Included: Cardiolipin Ab (IgA), Cardiolipin Ab (IgG), Cardiolipin Ab (IgM)
ABN Requirement:
          Preferred: Sodium Citrate Plasma
          Alternate: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Sodium Citrate (Light Blue Top) Tube
Gel-barrier (SST, Tiger Top) Tube


Sodium Citrate Plasma:

  1. Collect and label sample according to standard protocols. Fill sodium citrate tube to the fill immediately after draw. DO NOT SHAKE.
  2. Centrifuge for 10 minutes.
  3. Immediately aliquot sodium citrate plasma into transport tube labeled as “NaCit Plasma” and cap rightly. Discard original tube.


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store specimen at 2-8°C after collection and ship the same day per packaging instructions included with the provided shipping box.


Ambient (15-25°C): 4 days
Refrigerated (2-8°C):
7 days
Frozen (-20°C):
30 days

Causes for Rejection: Specimens other than sodium citrate plasma or serum; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia

Methodology: Immunoassay (IA)

Turn Around Time: 2 to 3 days

Reference Range:

Cardiolipin Ab, IgA (APL)Cardiolipin Ab, IgG (GPL)Cardiolipin Ab, IgM (MPL)Interpretation
21-8021-8021-80Low to medium positive
>80>80>80High positive

Clinical Significance: Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly systemic lupus erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis, and primary Sjögren’s syndrome.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.