Order Code: C316
ABN Requirement: No
Synonyms: E2; 17β-estradiol; Oestradiol
Volume: 0.5 mL
Minimum Volume: 0.2 mL
Container: Gel-barrier tube (SST, Tiger Top)
- Collect and label sample according to standard protocols.
- Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
- Allow blood to clot 30 minutes.
- Centrifuge for 10 minutes.
Special Instructions: Samples should not be taken from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last dose.
Please Note: Samples taken from patients being treated with Fulvestrant may show falsely increased estradiol levels.
Transport:Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.
Ambient (15-25°C): not acceptable
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 6 months
Deep Frozen (-70°C): 6 months
Causes for Rejection: Samples which are heat-inactivated; samples stabilized with azide; specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits
Methodology: Electrochemiluminescence Immunoassay (ECLIA)
Turn Around Time: 1 to 5 days
|All Ages, Male||27-52|
|Pregnancy, 1st Trimester||154-3065|
|Pregnancy, 2nd Trimester||1561-18950|
|Pregnancy, 3rd Trimester||10030- >30000|
Clinical Significance: An estradiol test is used to evaluate ovarian and adrenal gland function, as well to determine the cause of amenorrhea. It is also used for the diagnosis of ovarian cancer, and to monitor follicle development during in vitro fertilization and hormone replacement therapy.
Limitations: Erroneous test results may be obtained from samples taken from patients who have been exposed to vaccines containing rabbit serum or when keeping rabbits as pets. In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin, or ruthenium can occur. Samples taken from patients being treated with Fulvestrant may show falsely increased estradiol levels due to cross-reactivity.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.