Cleveland Heartlab F, Inflammation, Test


CPT Code: 82542/82570
Order Code: C261
Includes: F2-isoprostane, Urine Creatinine
ABN Requirement:  No
Specimen: Preservative-free Urine
Volume: 2.0 mL
Minimum Volume:  1.5 mL
Container: Yellow Top Urine Tube (No Preservative)


  1. Collect urine sample in a preservative-free urine collection container (cup) according to standard protocols.
  2. Transfer aliquot into a preservative-free urine specimen tube using the vacutainer transfer device included with the tube.
  3. Label urine specimen tube according to standard protocols. Store and transport refrigerated. Discard urine collection container (cup).

Transport: Store urine at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.


Ambient (15-25°C): 7 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 6 months

Causes for Rejection: Specimens other than preservative-free urine; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Liquid Chromatography, Tandem Mass Spectrometry (LC-MS/MS)

Turn Around Time: 5 days

Relative Risk Range:

AgeLow Relative Risk
(ng/mg creatinine)
High Relative Risk
(ng/mg creatinine)
All Ages<0.86≥0.86

Elevated urinary F2-Isoprostanes are associated with an increased risk of coronary heart disease (CHD) (1). (Reference: 1-Schwedhelm et al. Circulation. 2004; 109: 843-848.)

Clinical Significance: The F2-isoprostane/creatinine is the “gold standard” for measuring oxidative stress and has utility in individuals who have lifestyle risks due to poor diet or smoking, a family history of cardiovascular disease, or hyperlipidemia. High levels are seen in conditions associated with increased risk of atherosclerosis and certain cancers.

Limitations: Conditions that result in excessive generation of free radicals, including atherosclerosis, smoking and alcoholism, can result in increased levels of urinary isoprostanes.

Additional Information: The measurement of urinary isoprostanes is also used to assess efficacy of antioxidants in vivo.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.