Infectious Mono Slide Test (Monospot Test)

Cleveland Heartlab I, M, Test

CPT Code: 86308
Order Code: C2389
ABN Requirement: No
Synonyms: Monospot Test; Infectious Mono Slide Test; Heterophile Antibody; Heterophile Agglutination
Specimen: Serum
Volume:  0.5 mL
Minimum Volume:  0.3 mL
Container: Gel-barrier tube (SST, Tiger Top)



  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Let tube stand in a vertical position to allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.

Transport: Store serum at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.


Ambient (15-25°C): 48 hours
Refrigerated (2-8°C):
14 days
Frozen (-20°C):
1 year
Deep Frozen (-70°C):
1 year

Causes for Rejection: Specimens other than serum; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Latex Particle Agglutination (LPA)

Turn Around Time: 2-3 days

Reference Range:

AgeNormal Range
All AgesNegative

Use: Tests for antibodies to Infectious Mononucleosis.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.