Protein Electrophoresis, Urine Random

Cleveland Heartlab P, Test

CPT Code: 84156, 84166
Order Code: C2716
Test Includes: Urine total protein; % Albumin; % Alpha 1 globulin; % Alpha 2 globulin; % Beta globulin; % Gamma globulin; Interpretation
ABN Requirement: No
Synonyms: UPE; Bence Jones Protein Screen
Specimen: Random Urine or 24-Hour Urine (well-mixed)
Volume:  10.0 mL
Minimum Volume:  5.0 mL
Container: Urine (Yellow Top, no preservative) tube


  1. Collect sample according to standard protocols.
  2. Transfer aliquot from a clean urine cup or well-mixed urine from a 24-hour urine container into a Yellow Top tube using the vacutainer transfer device included with the Yellow Top tube.
  3. Gently invert tube 5 times
  4. Label sample according to standard protocols.

Special Information: First morning void is preferred.

Transport: Store urine at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.


Ambient (15-25°C): 2 hours
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 30 days

Causes for Rejection: Specimens other than random urine; urine collected in acid container; improper labeling; samples not stored properly; samples older than stability limits

Methodology: Electrophoresis

Turn Around Time: 1 to 7 days

Reference Range:

Age Reference Range
All Ages No evidence of M-Spike

Clinical Significance:  The random urine protein electrophoresis test is used to evaluate individuals with renal disorders, and to screen for free monoclonal immunoglobulin light chains (Bence Jones proteins).

Additional Information: A trace of albumin is commonly seen in normal patients. Monoclonal free immunoglobulin light chains and/or intact immunoglobulins may be detected in individuals with multiple myeloma and other B-cell lymphoproliferative disorders.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.