Testosterone, Total, MS

Cleveland HeartLab T, Test


CPT Code: 84403
Order Code: 15983
ABN Requirement: No
Specimen: Serum
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: Red Top (no gel barrier) tube


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into a transport tube labeled “Red Top Serum” and cap tightly.

*Special Information: Please specify age and sex on the test requisition form.*

Transport: Store serum at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.


Ambient (15-25°C): 7 days
Refrigerated (2-8°C):
21 days
Frozen (-20°C):
2 years

Causes for Rejection: Specimens other than Red Top serum; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia; specimens collected in Serum Separator Tube (SST)

Methodology: Chromatography/Mass Spectrometry

Turn Around Time:  3 to 5 days

Reference Range:

AgeMales (ng/dL)Females (ng/dL)
Cord Blood*17-6116-44
1-10 Days*≤187≤24
11-30 DaysNot EstablishedNot Established
1-2 Months*72-344≤17
3-4 Months*≤201≤12
5-6 Months*≤59≤13
7-11 Months*≤16≤11
1-5 Years≤5≤8
6-7 Years≤25≤20
8-10 Years≤42≤35
11 Years≤260≤40
12-13 Years≤420≤40
14-17.9 Years≤1000≤40
≥18 Years250-11002-45

*Data from J Clin Invest 1974;53:819-828 and J Clin Endocrinol Metab 1973;36:1132-1142

Clinical Significance: Helpful in assessing testicular function in males and managing hirsutism, virilization in females.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.