Vitamin B6

Cleveland Heartlab Test, V


CPT Code: 84207
Order Code: 926
Alternative Name(s): Pyridoxine, Pyridoxal Phosphate
ABN Requirement: No
Specimen: EDTA plasma, frozen
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container: EDTA (Lavender Top) tube – frozen plasma, protected from light


  1. Collect and label sample according to standard protocols.
  2. Gently invert EDTA tube 10 times immediately after draw. Do not shake.
  3. Centrifuge specimen for 8-10 minutes, within 6 hours of collection. (use of refrigerated centrifuge to separate cells at 2-8 °C preferred)
  4. Transfer plasma to an amber polypropylene or polyethylene transport tube labeled as “EDTA Plasma” to protect from light. Alternatively, neutral color polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Freeze tubes at -10 to -30°C immediately. Ship frozen.

Fasting: Overnight fasting is required

Transport: Store specimen at -10 to -30°C after collection and ship the same day per packaging instructions included with the provided shipping box.

  • Ship frozen specimen on dry ice


Ambient (15-25°C): 72 hours
Refrigerated (2-8°C):
5 days
Frozen (-20°C):
14 days
Deep Frozen (-70°C):
42 days

Causes for Rejection: Improper labeling; samples not stored properly; samples older than stability limits; hemolysis; lipemia; samples received not protected from light; samples received ambient or refrigerated

Methodology: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Turn Around Time:  3 to 5 days

Reference Range:

0-1 yearNot Established
2-17 years3.0-35.0
≥18 years2.1-21.7

Clinical Significance: Test is used to determine vitamin B6 deficiency or overdosage, for monitoring treatment, and to evaluate wellness and health.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.