Pregnenolone, LC/MS

Cleveland Heartlab P, Test

CPT Code: 84140
Order Code: 1200
ABN Requirement: No
Specimen: Serum from Red Top tube only
Volume:  0.5 mL
Minimum Volume:  0.2 mL
Container: Red Top (no gel barrier) tube


  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes at room temperature.
  4. Centrifuge for 10 minutes.
  5. Aliquot serum into transport tube labeled as “Red Top Serum” and cap tightly. Discard original tube.

Special Instructions:  A separate Red Top (no gel barrier) serum tube is needed for Pregnenolone, LC/MS. Collect additional samples for other testing that requires Red Top (no gel barrier) serum. Freeze serum immediately and ship with dry ice.

Transport:  Store serum frozen at -20°C after collection and ship on dry ice per packaging instructions included with the provided shipping box.


Ambient (15-25°C): Unacceptable
Refrigerated (2-8°C): 72 hours
Frozen (-20°C): 14 days
Deep Frozen (-70°C): 30 days

Causes for Rejection: Samples received at room temperature; serum from an SST tube; improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis

Methodology: Liquid Chromatography Mass Spectrometry (LC/MS)

Turn Around Time: 2-6 days

Reference Range:

Age ng/dL
1 day-59 days 68-1303
60 days-1 year ≤219
2-6 years ≤140
7-9 years ≤156
10-12 years 15-220
13-17 years 12-196
≥18 years (Adult) 22-237

Clinical Significance: A pregnenolone test can be used as an ancillary test for the diagnosis of congenital adrenal hyperplasia, and to confirm a diagnosis of 3-beta-hydroxy dehydrogenase deficiency.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.